Journal: Cell reports. Medicine

Article Title: DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription.

doi: 10.1016/j.xcrm.2025.102146

Figure Lengend Snippet: Figure 7. IM prevents AAD resistance, while co-treatment with IM and NTZ prevents metastasis and improves prognosis (A) The upregulated and downregulated differentially expressed genes between shADAM10-expressing CNE2 cells and control cells. (B) Molecular docking of IM with DDX5. Up: Libdock; down: CDOCKER. (C) Western blot analysis of DDX5, ADAM10, and EMT-related markers in CNE2 cells treated with metastasis-exo or IM alone, or their combination (3 independent experiments). (D) Kaplan-Meier analysis of overall survival (log rank test, n = 15 per group). (E) Metastasis of nasopharyngeal carcinoma orthotopic xenografts of orthotopic mouse model was visualized using BLI, on day 14 (n = 15 per group). (F) Overall metastasis rate of each group (n = 15 per group). (G) Kaplan-Meier analysis of overall metastasis (log rank test, n = 15 per group). (H and I) Immunohistochemistry analysis of PAS and CD31 double staining of orthotopic xenografts and ADAM10 and EMT-related marker expression in them. Quantification of VM, MV, and VM + MV numbers (H, n = 3 per group) and immunohistochemistry (IHC) staining scores (I, n = 6 per group). Two-way ANOVA, mean ± SEM, ns indicates p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. IM, ingenol mebutate; NTZ, nimotuzumab.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ingenol-Mebutate MCE Cat#HY-B0719 GW4869 MCE Cat#HY-19363 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Scientific Cat#23225 NovoNGS CUT&Tag 2.0 High-Sensitivity Kit Novoprotein Scientific Cat#N259-YH01–01A Epi HiC kit Epibiotek Cat#R1817 Epi DNA Clean Beads Epibiotek Cat#R1809 QIAseq Ultralow Input Library Kit Qiagen Cat#180495 Renilla-Firefly Luciferase Dual Assay Kit MCE Cat#HY-K1013 Agarose ChIP Kit Merck Cat#17–295 Deposited data CUT&Tag sequencing of NP69 and CNE2 This paper GEO: GSE263067 Hi-C data of NP69 and CNE2 This paper GEO: GSE263286, GSE283675 DIA mass spectrometry-based proteomics data This paper iProX: PXD052241 Experimental models: Cell lines CNE1 Kindly provided by Xiang-Ya School of Medicine N/A CNE2 Kindly provided by Xiang-Ya School of Medicine N/A 5-8F Kindly provided by Sun Yat-sen University N/A 6-10B Kindly provided by Xiang-Ya School of Medicine N/A C666-1 Kindly provided by Sun Yat-sen University N/A HK1 Kindly provided by Xiang-Ya School of Medicine N/A HUVEC Kindly provided by Sun Yat-sen University N/A Experimental models: Organisms/strains Mouse: male BALB/c nude mice GemPharmatech Co. Cat#D000521 Oligonucleotides Sequences of the siRNAs This paper Table S3 Primers used in quantitative real-time PCR This paper Table S4 Primers used in the Chip-qPCR analysis This paper Table S5 Recombinant DNA Sequences of the cas9 lentiviruses and plasmids This paper Table S2 Software and algorithms Graphpad Prism 6.0 GraphPad version 6.0 Connectivity Map (CMap) Clue.io https://clue.io ImageJ ImageJ https://imagej.nih.gov/ij SpectronautTM 14.4.200727.47784 SpectronautTM 14.4.200727.47784 Cell Reports Medicine 6, 102146, June 17, 2025 e2 Article ll OPEN ACCESS University.

Techniques: Expressing, Control, Western Blot, Immunohistochemistry, Double Staining, Marker

Journal: Cell reports

Article Title: CDK14 inhibition reduces mammary stem cell activity and suppresses triple negative breast cancer progression.

doi: 10.1016/j.celrep.2022.111331

Figure Lengend Snippet: Figure 6. FMF suppresses tumor growth and metastasis of TNBC cell line-derived xenograft (A) Cell viability assay following treatment with abemaciclib (Abe) or FMF for 48 h in MDA-MB-231 cells. n = 6. (B) Illustration of the experimental design for the Abe or FMF treatment of MDA-MB-231 tumors. MDA-MB-231 cells were orthotopically inoculated into recipient nude mice; when the tumor volume is approximately 50–100 mm3, mice given (orally) FMF or Abe at the dosage of 40 mg/kg body weight for 15 d, after which the mice were sacrificed (n = 5 mice). (C) Average tumor volume at the indicated days postAbe or FMF administration. (D) Individual tumor volume at the indicated days post-Abe or FMF administration. (E) Tumor weight and image of MDA-MB-231 tumors following treatment with Abe or FMF.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Abemaciclib MCE Cat#HY-16297 Puromycin Merck Cat#540411 DAPI Thermo Fisher Cat#P36931 RNAiso plus Takara Cat#9109 Critical commercial assays PrimeScript RT Master mix kit Takara Cat#RR036A FastStart Universal SYBR Green Master Mix kit Monad Cat#MQ10101S Nuclear and Cytoplasmic Protein Extration Kit Beyotime Cat#P0027 Endofree plasmid kit Tiangen Biotech Cat#DP118-02 Click-iT EdU Imaging Kits Invitrogen Cat#C10339 Annexin V Alexa Fluor 647/PI Apoptosis Detection Kit 4A Biotech Cat#FXP023 ALDEFLUOR kit Stem Cell Technologies Cat#01700 Deposited data RNA-seq This study GSE201526 RNA-seq This study GSE197213 RNA-seq This study GSE197000 Experimental models: Cell lines Human: HEK293T Shanghai Cell Bank Type Culture Collection Committee CSTR:19,375.09.3101HUMGNHu17 Human: MDA-MB-231 Shanghai Cell Bank Type Culture Collection Committee CSTR:19375.09.3101HUMTCHu227 Human: MDA-MB-468 Shanghai Cell Bank Type Culture Collection Committee CSTR:19375.09.3101HUMTCHu136 Human: MCF-7 Shanghai Cell Bank Type Culture Collection Committee CSTR:19375.09.3101HUMTCHu74 Mouse: 4T1 Shanghai Cell Bank Type Culture Collection Committee CSTR:19375.09.3101MOUTCM32 Mouse: Eph4 Gift of Prof. Yi Arial Zeng N/A Mouse: primary mammary epithelial cell This paper N/A Experimental models: Organisms/strains Mouse: BALB/c Charles River Laboratories N/A Mouse: BALB/c Nude Gem Pharmatech N/A Mouse: SCID/Beige Charles River Laboratories N/A Mouse: MMTV-Wnt1 Gift of Prof. Yi Arial Zeng N/A Oligonucleotides See Table S1 N/A N/A Recombinant DNA pLKO.1-EGFP Addgene Cat#32684 pLKO.1-hCDK14-EGFP This study N/A pLKO.1-mCdk14-EGFP This study N/A pLKO.1-mCherry Addgene Cat#128073 pLKO.1-mCdk14-mCherry This study N/A pWPI-EGFP Addgene Cat#12254 pWPI-CDK14-EGFP This study N/A pWPI-b-cateninDEx3-EGFP This study N/A pLVX-puro youbio Cat#VT1464 pLVX-CDK14-puro This study N/A (Continued on next page) Cell Reports 40, 111331, September 13, 2022 e2

Techniques: Derivative Assay, Viability Assay